2PFB
Structure of oxidized OhrR from Xanthamonas campestris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-13 |
| Detector | RIGAKU RAXIS HTC |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.140, 98.260, 88.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.760 - 1.930 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | residues 18 to 107 from pdb entry 2PEX |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.760 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.062 | 0.280 |
| Number of reflections | 20477 | |
| <I/σ(I)> | 14 | 4.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.5 | 6.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% PEG 4000, 300mM magnesium chloride, 100 mM Tris, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
