2PF6

Lutheran glycoprotein, N-terminal domains 1 and 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.488
Spacegroup name	P 32 2 1
Unit cell lengths	52.248, 52.248, 256.842
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	85.750 - 2.200
R-factor	0.207
Rwork	0.205
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pet
RMSD bond length	0.018
RMSD bond angle	1.498
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.102	0.482
Number of reflections	19830
<I/σ(I)>	10	1.2
Completeness [%]	90.1	41.3
Redundancy	5.6	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	18% PEG4000, 0.2M ammonium acetate, 0.1M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K