2PF2

THE CA+2 ION AND MEMBRANE BINDING STRUCTURE OF THE GLA DOMAIN OF CA-PROTHROMBIN FRAGMENT 1

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	39.390, 53.880, 129.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.171
RMSD bond length	0.017
RMSD bond angle	0.055
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Number of reflections	8141 *
Completeness [%]	64.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *		taken from Soriano-Garacia, M. et al (1989), Biochemistry, 28, 6805-6810. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	cacodylate	0.1 (M)
2	1	drop	PEG6000	8 (%(w/v))
3	1	drop		100 (mM)
4	1	reservoir	PEG6000	16 (%(w/v))
5	1	reservoir	cacodylate	0.1 (M)
6	1	reservoir		200 (mM)