2PET

Lutheran glycoprotein, N-terminal domains 1 and 2.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX10.1
Synchrotron site	SRS
Beamline	PX10.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.285
Spacegroup name	P 1 21 1
Unit cell lengths	32.955, 111.062, 35.301
Unit cell angles	90.00, 90.45, 90.00

Refinement procedure

Resolution	55.640 - 1.700
R-factor	0.17
Rwork	0.168
R-free	0.21000
Structure solution method	MIRAS
RMSD bond length	0.015
RMSD bond angle	1.678
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE (CCP4_5.99)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.640	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.059	0.264
Number of reflections	24823
<I/σ(I)>	13.3	2.49
Completeness [%]	89.1	48.3
Redundancy	6.4	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	291	12% PEG 20000, 6% heaxane-1,2-diol, 0.1M MES, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.4	291	12% PEG 20000, 6% heaxane-1,2-diol, 0.1M MES, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.4	291	12% PEG 20000, 6% heaxane-1,2-diol, 0.1M MES, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.4	291	12% PEG 20000, 6% heaxane-1,2-diol, 0.1M MES, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K