2PD7
2.0 Angstrom Crystal Structure of the Fungal Blue-Light Photoreceptor Vivid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.350, 80.700, 58.570 |
| Unit cell angles | 90.00, 95.76, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.285 |
| Rwork | 0.245 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g28 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.720 | 0.202 |
| Number of reflections | 20707 | |
| <I/σ(I)> | 22.56 | 4.32 |
| Completeness [%] | 92.3 | 66.4 |
| Redundancy | 3.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 30% PEG 5000 MME, 0.1M Ammonium Acetate, 0.1M tri-sodium Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
