2PD1
Crystal structure of NE2512 protein of unknown function from Nitrosomonas europaea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.308, 68.137, 162.645 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.900 - 1.860 |
| R-factor | 0.1598 |
| Rwork | 0.158 |
| R-free | 0.18540 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.553 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.900 | 1.910 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.109 | 0.617 |
| Number of reflections | 31503 | |
| <I/σ(I)> | 24.4 | 2.11 |
| Completeness [%] | 98.6 | 86.9 |
| Redundancy | 13 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.2M Na Acetate, 0.15M Bis-Tris pH 6.5, 36% PEG8000, 2% Dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
