2PCD

STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE FROM PSEUDOMONAS AERUGINOSA AT 2.15 ANGSTROMS RESOLUTION

Replaces:  1PCD

Experimental procedure

Spacegroup name	I 1 2 1
Unit cell lengths	197.170, 127.030, 134.180
Unit cell angles	90.00, 97.64, 90.00

Refinement procedure

Resolution	5.000 - 2.150
R-factor	0.172
RMSD bond length	0.017
RMSD bond angle	0.030
Refinement software	PROLSQ

Data quality characteristics

	Overall
Number of reflections	164157
Completeness [%]	95.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	free interface diffusion *	8.4 *	4 *	or hanging drop vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20-75 (mg/ml)
2	1	2	ammonium sulfate	1.5-1.8 (M)
3	1	2	Tris-HCl	50 (mM)
4	1	2	beta-mercaptoethanol	10 (mM)