2PC6

Crystal structure of putative acetolactate synthase- small subunit from Nitrosomonas europaea

Replaces:  2FGD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97950
Spacegroup name	P 42 21 2
Unit cell lengths	122.123, 122.123, 111.559
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.580 - 2.500
R-factor	0.20846
Rwork	0.205
R-free	0.27573
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.838
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.580	2.570
High resolution limit [Å]	2.500	2.500
Rmerge	0.084	0.431
Number of reflections	28271
<I/σ(I)>	60.72	7.35
Completeness [%]	99.9	100
Redundancy	18.9	19.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	2% TACSIMATE, 10% PEG 400, 0.1M KCl, 10mM CaCl2, 50 mM Na-HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K