2PC4
Crystal structure of fructose-bisphosphate aldolase from Plasmodium falciparum in complex with TRAP-tail determined at 2.4 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.386, 145.519, 148.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.400 |
| R-factor | 0.20088 |
| Rwork | 0.198 |
| R-free | 0.24983 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a5c |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.095 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.970 | 2.678 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 54265 | |
| <I/σ(I)> | 11.7 | 2.3 |
| Completeness [%] | 94.7 | 84.84 |
| Redundancy | 3.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 2.5 mM TRAP-tail, 0.5% n-dodecyl-beta-D-maltoside, 50 mM Cacodylic acid pH 6.0, 20% PEG 2000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
