2PC0
Apo Wild-type HIV Protease in the open conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2006-02-01 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 46.416, 46.416, 101.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.800 - 1.400 |
| R-factor | 0.155 |
| Rwork | 0.153 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2az8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.196 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.800 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 22409 | |
| <I/σ(I)> | 16.5 | 3 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 6.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 277 | 0.2 M Magnesium chloride, 20% w/v PEG 3350, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
