2PBY
Probable Glutaminase from Geobacillus kaustophilus HTA426
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.503, 86.786, 106.881 |
| Unit cell angles | 90.00, 109.76, 90.00 |
Refinement procedure
| Resolution | 19.860 - 2.070 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mki |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.676 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR (2.5) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.177 | |
| Number of reflections | 75938 | |
| <I/σ(I)> | 18.1 | 7.38 |
| Completeness [%] | 93.5 | 88.3 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 291 | 20% PEG 4000, 0.08 M Na Acetate pH 4.6, 0.16 M Ammonium Sulfate, 20% Glycerol, VAPOR DIFFUSION, temperature 291K |
