2PBN
Crystal structure of the human tyrosine receptor phosphate gamma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.10000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.338, 107.338, 67.757 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.209 |
| Rwork | 0.208 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hy3 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.527 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.683 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.054 | 0.645 |
| Number of reflections | 49677 | |
| <I/σ(I)> | 16.1 | 2.2 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 10.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | 100mM Sodium MES pH 6.5, 22% PEG 8000, 200mM Ammonium sulfate, VAPOR DIFFUSION, temperature 294K |
