2PBG

6-PHOSPHO-BETA-D-GALACTOSIDASE FORM-B

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1994-08
Detector	SIEMENS
Spacegroup name	C 2 2 21
Unit cell lengths	104.000, 179.600, 60.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.182
Rwork	0.182
R-free	0.29400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pbg
RMSD bond length	0.009
RMSD bond angle	24.500 *
Data reduction software	XDS
Data scaling software	BIOMOL
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	1000.000	2.500
High resolution limit [Å]	2.500	2.430
Rmerge	0.096 *
Number of reflections	16050
Completeness [%]	79.0	50
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop		0.1 (M)
3	1	drop		0.02 (%)
4	1	reservoir	PEG4000	28 (%)
5	1	reservoir		0.2 (M)
6	1	reservoir	Tris-HCl	0.1 (M)
7	1	reservoir		0.02 (%)