2PBC
FK506-binding protein 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-23 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.430, 39.335, 108.316 |
| Unit cell angles | 90.00, 91.28, 90.00 |
Refinement procedure
| Resolution | 23.060 - 1.800 |
| R-factor | 0.18861 |
| Rwork | 0.185 |
| R-free | 0.25986 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y0o |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.687 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.100 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 32970 | |
| <I/σ(I)> | 27.8 | 7.8 |
| Completeness [%] | 98.5 | 85.3 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 32% PEG MME550, CRYOPROTECTED IN PARATONE-N, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
