2PB8
Crystal structure of the complex formed between phospholipase A2 and peptide Ala-Val-Tyr-Ser at 2.0 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54132 |
| Spacegroup name | P 43 |
| Unit cell lengths | 53.087, 53.087, 48.364 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.450 - 2.000 |
| R-factor | 0.17924 |
| Rwork | 0.177 |
| R-free | 0.21804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sv3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.849 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.450 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 8317 | |
| Completeness [%] | 98.3 | 90.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | Acetate, 30% PEG4000, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
