2PAJ
Crystal structure of an amidohydrolase from an environmental sample of Sargasso sea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.872, 88.872, 161.867 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.440 - 2.700 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.28600 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.070 | 0.300 |
| Number of reflections | 19968 | |
| <I/σ(I)> | 11.1 | 1 |
| Completeness [%] | 94.4 | 63 |
| Redundancy | 19 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M MES pH 6.5, 12% PEG 20000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
