2PAF
Crystal Structure of the Lactose Repressor bound to anti-inducer ONPF in induced state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-06 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 77.541, 77.541, 212.456 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.810 - 3.500 |
| R-factor | 0.19856 |
| Rwork | 0.195 |
| R-free | 0.26516 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1efa |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.805 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.166 | 0.336 |
| Number of reflections | 8685 | |
| <I/σ(I)> | 5.9 | 3.5 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 281 | 1.4 M Sodium Acetate, 0.1 M Sodium Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
