2PA6
Crystal structure of MJ0232 from Methanococcus jannaschii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-02-11 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | I 4 |
Unit cell lengths | 148.783, 148.783, 91.197 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.880 - 1.850 |
R-factor | 0.17 |
Rwork | 0.170 |
R-free | 0.18600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oep |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.078 | 0.529 |
Number of reflections | 85037 | |
<I/σ(I)> | 15.4 | 4.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.5 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 4.6 | 291 | 0.1M Sodium Acetate, 8% PEG4000, pH4.6, MICROBATCH, temperature 291K |