2P9X
Crystal structure of PH0832 from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-05 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.357, 65.870, 104.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 1.650 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.24500 |
| Structure solution method | MAD |
| RMSD bond length | 1.100 |
| RMSD bond angle | 0.005 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.059 | 0.627 |
| Number of reflections | 52277 | |
| <I/σ(I)> | 10.9 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | oil-micro batch | 5.8 | 295 | 10% isopropanol, 0.1M cacodylate, 0.2M zinc acetate, pH 5.8, oil-micro batch, temperature 295K |
