2P9T
Crystal Structure of Phosphoglycerate Kinase-2 bound to 3-phosphoglycerate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-05 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.100, 64.300, 72.200 |
| Unit cell angles | 90.00, 97.80, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vjd |
| RMSD bond length | 0.007 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.209 |
| Number of reflections | 24578 | |
| <I/σ(I)> | 14.6 | |
| Completeness [%] | 99.3 | 96.7 |
| Redundancy | 6.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 10 mM D-3-phosphoglycerate, 30% PEG6000, 0.1 M Tris-Hcl, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 8.00 |
