2P9I

Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.008
Spacegroup name	P 21 21 21
Unit cell lengths	110.610, 128.184, 198.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.460
R-factor	0.219
Rwork	0.217
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.520
High resolution limit [Å]	2.430	2.430
Rmerge	0.063	0.542
Number of reflections	105282
<I/σ(I)>	11.2	2.4
Completeness [%]	98.4	96.4
Redundancy	4.1	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	7.5% PEG 3350 50mM HEPES 100mM KSCN 10% Sucrose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K