2P9G
Crystal structure of serine bound G336V,G337V double mutant of E.coli phosphoglycerate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 144.072, 132.239, 52.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.800 |
| R-factor | 0.20004 |
| Rwork | 0.197 |
| R-free | 0.25008 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1psd |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.869 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.320 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.052 | 0.207 |
| Number of reflections | 25289 | |
| <I/σ(I)> | 20.1 | 4.8 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 30% PEG 400, 0.1M acetate, 0.2M Calcium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
