2P8B

Crystal structure of N-succinyl Arg/Lys racemase from Bacillus cereus ATCC 14579 complexed with N-succinyl Lys.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	I 4 2 2
Unit cell lengths	134.924, 134.924, 96.791
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.440 - 1.700
R-factor	0.206
Rwork	0.206
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2p88
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.700
Rmerge	0.081
Number of reflections	47638
Completeness [%]	97.0
Redundancy	15.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	18% PEG 3350, 0.1 M Succinic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K