2P84
Crystal structure of ORF041 from Bacteriophage 37
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-16 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.470, 109.370, 57.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.880 - 1.800 |
| R-factor | 0.212 |
| Rwork | 0.208 |
| R-free | 0.27300 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.816 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.668 | 1.920 |
| High resolution limit [Å] | 1.800 | 1.810 |
| Rmerge | 0.079 | 0.330 |
| Number of reflections | 16818 | |
| <I/σ(I)> | 11.2 | 1 |
| Completeness [%] | 98.5 | 100 |
| Redundancy | 13.6 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 295 | 0.1M HEPES, pH 7.5, 1.4 M tri-sodium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 295K, pH 7.50 |
