2P7S
Enzymatic and Structural Characterisation of Amphinase, a Novel Cytotoxic Ribonuclease from Rana pipiens Oocytes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-25 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 41 |
| Unit cell lengths | 53.967, 53.967, 47.796 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.200 - 1.800 |
| Rwork | 0.221 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1onc |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.494 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.200 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.069 | 0.354 |
| Number of reflections | 12605 | |
| <I/σ(I)> | 16 | |
| Completeness [%] | 98.0 | 86.5 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | protein 25 mg/ml, PEG 8000 30% (w/v), Na-acetate 0.2M, Na-cacodylate 0.1M, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
