2P7P
Crystal structure of genomically encoded fosfomycin resistance protein, FosX, from Listeria monocytogenes complexed with MN(II) and sulfate ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.500, 70.565, 84.145 |
| Unit cell angles | 90.00, 113.74, 90.00 |
Refinement procedure
| Resolution | 23.080 - 2.170 |
| Rwork | 0.226 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p7k |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.250 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Number of reflections | 46644 | |
| <I/σ(I)> | 14.7 | 2.1 |
| Completeness [%] | 97.1 | 85.8 |
| Redundancy | 2.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 30% PEG 8000, 50 mM Na cacodylate, 0.1 M (NH4)2SO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
