2P7O

Crystal structure of genomically encoded fosfomycin resistance protein, FosX, from Listeria monocytogenes (tetragonal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-04-14
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 43
Unit cell lengths	68.579, 68.579, 56.835
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.440
R-factor	0.126
Rwork	0.124
R-free	0.16700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2p7k
RMSD bond length	0.012
RMSD bond angle	0.034
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.460
High resolution limit [Å]	1.440	1.440
Number of reflections	41281
<I/σ(I)>	24.4	2.1
Completeness [%]	86.5	97.6
Redundancy	4.6	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	295	22% PEG 3350, 0.1 M Tris-HCl, 0.2 M MgCl2, 5% isopropanol, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K