2P7M
Crystal structure of monoclinic form of genomically encoded fosfomycin resistance protein, FosX, from Listeria monocytogenes at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.431, 69.693, 84.869 |
| Unit cell angles | 90.00, 113.98, 90.00 |
Refinement procedure
| Resolution | 18.620 - 1.850 |
| Rwork | 0.239 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p7k |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.620 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 68258 | |
| <I/σ(I)> | 23.1 | 1.8 |
| Completeness [%] | 88.4 | 60.8 |
| Redundancy | 2.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 18-20% PEG 8000, 0.1M Na citrate, 4-12 mM Na acetate, 10% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
