2P7L

Crystal structure of monoclinic form of genomically encoded fosfomycin resistance protein, FosX, from Listeria monocytogenes at pH 5.75

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-02-15
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	168.779, 70.697, 86.974
Unit cell angles	90.00, 113.98, 90.00

Refinement procedure

Resolution	27.700 - 2.200
Rwork	0.229
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2p7k
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.700	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	44743
<I/σ(I)>	23.1	2.2
Completeness [%]	95.5	73.4
Redundancy	3.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.75	295	18-20% PEG 8000, 0.1M Na citrate, 4-12 mM Na acetate, 10% glycerol, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 295K