2P7G
X-ray Structure of Estrogen Related Receptor g in complex with Bisphenol A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2003-08-06 |
| Detector | BRUKER SMART 6000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.063, 64.063, 136.484 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.500 - 2.100 |
| Rwork | 0.215 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kv6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.063 |
| Data reduction software | SAINT |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.500 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.048 | 0.140 |
| Number of reflections | 17455 | |
| <I/σ(I)> | 11.7 | 4.3 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 22.6% polyethylene glycol 4000, 0.1M Tris, pH 8.5 and 0.2M Sodium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
