2P7F
The Novel Use of a 2',5'-Phosphodiester Linkage as a Reaction Intermediate at the Active Site of a Small Ribozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97945 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 94.100, 94.100, 127.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.380 - 2.350 |
R-factor | 0.226 |
Rwork | 0.227 |
R-free | 0.25200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1ZFR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.760 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 14321 | |
<I/σ(I)> | 19.6 | 2.9 |
Completeness [%] | 99.1 | 99.1 |
Redundancy | 5.92 | 5.32 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 250 mM Li2SO4, 2.5 mM [Co(NH3)6]Cl3, 2 mM spermidine-HCl, 15 mM nicotinic acid, 100 mM sodium HEPES buffer and 20.5% PEG 2000 MME, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Li2SO4 | ||
10 | 1 | 2 | sodium HEPES | ||
2 | 1 | 1 | [Co(NH3)6]Cl3 | ||
3 | 1 | 1 | spermidine-HCl | ||
4 | 1 | 1 | nicotinic acid | ||
5 | 1 | 1 | sodium HEPES | ||
6 | 1 | 1 | PEG 2000 MME | ||
7 | 1 | 2 | Li2SO4 | ||
8 | 1 | 2 | spermidine-HCl | ||
9 | 1 | 2 | PEG 2000 MME |