2P73

crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-11-15
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	43.375, 63.535, 44.760
Unit cell angles	90.00, 114.03, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.193
Rwork	0.190
R-free	0.24300
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.011
RMSD bond angle	1.282
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.091	0.043	0.358
Number of reflections	9682
<I/σ(I)>	13.2
Completeness [%]	97.7	96.6	92.7
Redundancy	3.1	3.2	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1