2P72
crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.448, 61.776, 76.091 |
| Unit cell angles | 90.00, 126.56, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.208 |
| Rwork | 0.207 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.274 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 4.190 | 1.950 |
| Rmerge | 0.068 | 0.044 | 0.456 |
| Number of reflections | 32704 | ||
| <I/σ(I)> | 16.3 | ||
| Completeness [%] | 94.9 | 95.9 | 92.8 |
| Redundancy | 3.3 | 3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
