2P70

Bombyx mori pheromone binding protein bound to bell pepper odorant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Wavelength(s)	0.95
Spacegroup name	I 41
Unit cell lengths	83.850, 83.850, 34.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.760 - 2.100
R-factor	0.205
Rwork	0.203
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dqe
RMSD bond length	0.046
RMSD bond angle	2.918
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	59.760
High resolution limit [Å]	2.100
Rmerge	0.115
Number of reflections	6555
<I/σ(I)>	3.77
Completeness [%]	94.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	PEG 4000, Tris, MgSO4, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K