2P6Z
Enzymatic and Structural Characterisation of Amphinase, a Novel Cytotoxic Ribonuclease from Rana pipiens Oocytes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-12 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 1 |
Unit cell lengths | 29.795, 44.607, 46.054 |
Unit cell angles | 116.56, 83.19, 103.68 |
Refinement procedure
Resolution | 41.200 - 1.930 |
R-factor | 0.148 |
Rwork | 0.146 |
R-free | 0.19100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Amphinase-2 (native) |
RMSD bond length | 0.009 |
RMSD bond angle | 1.224 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.200 | 2.010 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmerge | 0.037 | 0.108 |
Number of reflections | 14385 | |
<I/σ(I)> | 18.8 | 7.5 |
Completeness [%] | 89.8 | 63.5 |
Redundancy | 2.1 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | protein: 12.5 mg/ml, PEG 4000 30%, Na Citrate 0.1 M, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |