2P6Y

X-ray structure of the protein Q9KM02_VIBCH from Vibrio cholerae at the resolution 1.63 A. Northeast Structural Genomics Consortium target VcR80.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-07
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97900
Spacegroup name	F 4 3 2
Unit cell lengths	172.924, 172.924, 172.924
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.970 - 1.630
R-factor	0.207
Rwork	0.207
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.690
High resolution limit [Å]	1.630	1.630
Number of reflections	51743
<I/σ(I)>	56	8.1
Completeness [%]	99.2	92.1
Redundancy	21.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	10 mM MgSO4, 50 mM Cacodylic acid, 200 mM (NH4)2SO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K