2P6W

Crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-08-15
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	43.229, 63.017, 44.772
Unit cell angles	90.00, 115.42, 90.00

Refinement procedure

Resolution	8.000 - 1.600
R-factor	0.175
Rwork	0.172
R-free	0.19500
RMSD bond length	0.010
RMSD bond angle	1.397
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.067	0.036	0.429
Number of reflections	31588
<I/σ(I)>	15.3
Completeness [%]	90.6	99.8	36.2
Redundancy	3.4	3.7	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	50mM socium citrate, 200mM NaCl, 25% PEG3500, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6	293	50mM socium citrate, 200mM NaCl, 25% PEG3500, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6	293	50mM socium citrate, 200mM NaCl, 25% PEG3500, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K