2P6V
Structure of TAFH domain of the human TAF4 subunit of TFIID
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9797, 1.0199 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 74.852, 74.852, 131.785 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.940 - 2.000 |
| R-factor | 0.21567 |
| Rwork | 0.214 |
| R-free | 0.24835 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.343 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.940 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.095 | 0.105 |
| Number of reflections | 15423 | |
| <I/σ(I)> | 16.7 | 3.7 |
| Completeness [%] | 98.5 | 98.3 |
| Redundancy | 13.9 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 295 | Crystals of methylated hTAF4-TAFH containing SeMet were grown by hanging drop vapor diffusion at 22 C by mixing equal volumes of protein solution and crystallization buffer (200 mM ammonium acetate, 2.2 M ammonium sulfate and 150 mM sodium bromide). , pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
