2P6U

Apo structure of the Hel308 superfamily 2 helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	136.150, 136.150, 230.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.140
Rwork	0.230
R-free	0.27600
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.656
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.140	3.140
Rmerge	0.086	0.755
Number of reflections	22283
<I/σ(I)>		62.2
Completeness [%]	46.5	94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.1 M NaCitrate pH 5.6; 1 M (NH4)2PO4; 15% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.1 M NaCitrate pH 5.6; 1 M (NH4)2PO4; 15% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K