2P6P

X-ray crystal structure of C-C bond-forming dTDP-D-Olivose-transferase UrdGT2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.1036
Spacegroup name	P 21 21 21
Unit cell lengths	65.872, 78.904, 133.958
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.040 - 1.880
R-factor	0.19028
Rwork	0.188
R-free	0.22978
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.584
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.040	68.000
High resolution limit [Å]	1.880	1.900
Rmerge	0.093
Number of reflections	57345
<I/σ(I)>	15.7	15.7
Completeness [%]	99.6	99.6
Redundancy	4.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	0.1M HEPES,18% PEG 1500, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	0.1M HEPES,18% PEG 1500, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	0.1M HEPES,18% PEG 1500, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K