2P69
Crystal Structure of Human Pyridoxal Phosphate Phosphatase with PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 54.559, 54.559, 212.537 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.270 - 2.250 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yoc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.361 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.090 | 0.825 |
| Number of reflections | 16163 | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 13 | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1 M Citric acid pH 5.5, 20% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
