2P67

Crystal structure of LAO/AO transport system kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X3A
Synchrotron site	NSLS
Beamline	X3A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-27
Detector	MAR CCD 165 mm
Wavelength(s)	0.9793
Spacegroup name	I 2 2 2
Unit cell lengths	56.311, 108.452, 141.296
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.190 - 1.800
R-factor	0.208
Rwork	0.207
R-free	0.23000
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.202
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.051	0.480
Number of reflections	40015
<I/σ(I)>	9.1	2.3
Completeness [%]	98.3	100
Redundancy	9.1	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	2.5 M NaCl, 10% MPD, 0.1M CAPS pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	9	298	2.5 M NaCl, 10% MPD, 0.1M CAPS pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	9	298	2.5 M NaCl, 10% MPD, 0.1M CAPS pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K