2P65
Crystal Structure of the first nucleotide binding domain of chaperone ClpB1, putative, (Pv089580) from Plasmodium Vivax
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.728, 52.213, 91.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.060 - 1.700 |
| R-factor | 0.20295 |
| Rwork | 0.201 |
| R-free | 0.23927 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1JBK.pdb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.074 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.060 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.340 |
| Number of reflections | 16520 | |
| <I/σ(I)> | 19.1 | 5.08 |
| Completeness [%] | 93.8 | 67.4 |
| Redundancy | 6.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.4 M Sodium Citrate 100 mM Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
