2P5S
RAB domain of human RASEF in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-19 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.275, 42.524, 75.656 |
| Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| Rwork | 0.211 |
| R-free | 0.26020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g16 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.423 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.077 | 0.032 | 0.751 |
| Number of reflections | 29262 | 3201 | 2125 |
| <I/σ(I)> | 8.1 | ||
| Completeness [%] | 94.9 | ||
| Redundancy | 3.4 | 3.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 20% PEG-3350, 0.2M KF, vapor diffusion, sitting drop |
