2P5M
C-terminal domain hexamer of AhrC bound with L-arginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 117.014, 117.014, 68.927 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.110 - 1.950 |
| R-factor | 0.187 |
| Rwork | 0.182 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b4b Trimer of C-terminal domains from B. steareothermophilus |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.415 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.110 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.077 | 0.391 |
| Number of reflections | 20787 | |
| <I/σ(I)> | 32.4 | 6.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 16.7 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 277 | 28% PEG 400, 0.1M Tris-HCl, 0.2M magnesium chloride, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
