2P5G
Crystal structure of RB69 gp43 in complex with DNA with dAMP opposite an abasic site analog in a 21mer template
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-03 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 131.628, 122.272, 163.871 |
Unit cell angles | 90.00, 96.45, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.24013 |
Rwork | 0.234 |
R-free | 0.29524 |
Structure solution method | Isomorphous Replacement |
Starting model (for MR) | 1RV2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.062 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.3.0011) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.085 | 0.402 |
Number of reflections | 125168 | |
<I/σ(I)> | 14.3 | 2.6 |
Completeness [%] | 98.5 | 87.7 |
Redundancy | 3.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.0, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 2000 MME | ||
2 | 1 | 1 | sodium acetate | ||
3 | 1 | 1 | magnesium sulfate | ||
4 | 1 | 1 | beta-mercaptoethanol | ||
5 | 1 | 2 | PEG 2000 MME | ||
6 | 1 | 2 | sodium acetate | ||
7 | 1 | 2 | magnesium sulfate |