2P58
Structure of the Yersinia pestis Type III secretion system needle protein YscF in complex with its chaperones YscE/YscG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97928 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.500, 94.540, 31.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.1901 |
| Rwork | 0.190 |
| R-free | 0.22140 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.101 | 0.367 |
| Number of reflections | 21105 | |
| <I/σ(I)> | 16.2 | 4.28 |
| Completeness [%] | 98.6 | 95.1 |
| Redundancy | 6.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Ammonium fluoride, 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
