2P57

GAP domain of ZNF289, an ID1-regulated zinc finger protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-09-28
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	41.027, 41.165, 72.808
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.19773
Rwork	0.196
R-free	0.24140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2crw
RMSD bond length	0.017
RMSD bond angle	1.371
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.060	0.028	0.504
Number of reflections	11721	1302	1129
<I/σ(I)>	15.9
Completeness [%]	97.8
Redundancy	5.4	5.1	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	3.2M sodium formate, .09M sodium acetate, pH 4.6, vapor diffusion, sitting drop, temperature 291K