2P56
Crystal structure of alpha-2,3-sialyltransferase from Campylobacter jejuni in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-07 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | I 4 |
| Unit cell lengths | 112.396, 112.396, 58.799 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1RO7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.191 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.057 | 0.236 |
| Number of reflections | 18129 | |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 96.5 | 66.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20mM Tris-HCl pH 7.5, and 200mM NaCl |
