2P4V
Crystal structure of the transcript cleavage factor, GreB at 2.6A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 43 |
| Unit cell lengths | 148.320, 148.320, 116.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.23 |
| Rwork | 0.226 |
| R-free | 0.25700 |
| Structure solution method | Single Wavelength |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.790 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.057 | 0.498 |
| Number of reflections | 74091 | |
| <I/σ(I)> | 32.6 | 2.8 |
| Completeness [%] | 95.3 | 73 |
| Redundancy | 6.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 3.75% PEG 8000, 25 mM ammonium sulfate, 12 mM sodium cacodylate, 8mM TRIS HCl, 800 mM NaCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
